Fig. 6. Effect of ITH12674 on photoreceptor connectivity. (A-H) Representative vertical central retinal sections of rd10 mice (A,C,E,G) and ITH12674-treated (10 mg/Kg) rd10 mice (B,D,F,H) (age P30) labeled for calbindin (green; A,B), CtBP2 (red; C,D), VGluT1 (blue; E,F) and merged images (G,H). In ITH12674-treated animals, immunolabeling showed horizontal cells with longer and more horizontal dendritic tips (Calbindin), a higher number of ribbon synapsis (higher density of CtBP2 spots showing the typical horse-shoes morphology) and a continuous OPL layer with still functional photoreceptor axon-terminals (VGluT1 staining). The merged image shows higher density of complete synaptic connections (H, arrowheads).